Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | … was used to generate the overexpressing vector (GenScript, Piscataway, New Jersey, USA). … Overall, 20 μg of proteins were loaded and separated in a NuPAGE Novex 4–12% Bis-Tris … | Get A Quote |
Glioblastoma (GBM) is the most aggressive primary brain tumor characterized by infiltrative growth of malignant glioma cells into the surrounding brain parenchyma. In this study, our analysis of GBM patient cohorts revealed a significantly higher expression of 8 () compared to normal brain tissue and could be associated with impaired patient survival. Increased expression of significantly enhanced migration of two different sphere-forming GBM cell lines. By analysis we predicted the 3D-structure as well as the active site residues of GLT8D1. The introduction of point mutations in the predicted active site reduced its glycosyltransferase activity and consequently impaired GBM tumor cell migration. Examinat... More