Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … The pET16b-10×HIS-CPE plasmid was generated by GenScript 103,104 . An overnight culture (10 ml) of BL21-Codon Plus (DE3)-RIL competent cells (Agilent Technologies, 230245) … | Get A Quote |
The contribution of the epicardium, the outermost layer of the heart, to cardiac regeneration has remained controversial due to a lack of suitable analytical tools. By combining genetic marker-independent lineage-tracing strategies with transcriptional profiling and loss-of-function methods, we report here that the epicardium of the highly regenerative salamander species Pleurodeles waltl has an intrinsic capacity to differentiate into cardiomyocytes. Following cryoinjury, CLDN6 epicardium-derived cells appear at the lesion site, organize into honeycomb-like structures connected via focal tight junctions and undergo transcriptional reprogramming that results in concomitant differentiation into de novo cardiomyo... More