Products/Services Used | Details | Operation |
---|---|---|
Custom Vector Construction> | … ) and CPSF73 (NM_016207.4) were purchased from Genscript. pSBtet-GP was a gift from Eric Kowarz … pCMV(CAT)T7-SB100 was used as a helper vector to supply the Sleeping Beauty … | Get A Quote |
Cleavage by the endonuclease CPSF73 and polyadenylation of nascent RNA is an essential step in co-transcriptional mRNA maturation. Recent work has surprisingly identified CPSF73 as a promising drug target for inhibiting the growth of specific cancers, triggering further studies on understanding CPSF73 regulation and functions in cells. Here, we report that a HECT-like E3 ligase, UBE3D, participates in stabilizing CPFS73 protein by preventing its ubiquitin-mediated degradation by the proteasome. Depletion of leads to CPSF73 downregulation, a pre-mRNA cleavage defect, and dysregulated gene expression in cells. UBE3D dysfunction or chemical inactivation of CPSF73 inhibited migration and invasion as well as stem c... More