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Biotransformation of Benzoate to 2,4,6-Trihydroxybenzophenone by Engineered

Molecules. 2021-05; 
Anuwatchakij Klamrak, Jaran Nabnueangsap, Natsajee Nualkaew
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ORF cDNA Clones/MolecularCloud The map of pETDuet-BadA-GmBPS (8069 bp), created by using GenScript (https://www.genscript.com/gensmart-design/#  Get A Quote

摘要

The synthesis of natural products by is a challenging alternative method of environmentally friendly minimization of hazardous waste. Here, we establish a recombinant capable of transforming sodium benzoate into 2,4,6-trihydroxybenzophenone (2,4,6-TriHB), the intermediate of benzophenones and xanthones derivatives, based on the coexpression of benzoate-CoA ligase from (BadA) and benzophenone synthase from (GmBPS). It was found that the engineered accepted benzoate as the leading substrate for the formation of benzoyl CoA by the function of BadA and subsequently condensed, with the endogenous malonyl CoA by the catalytic function of BPS, into 2,4,6-TriHB. This metabolite was excreted into the culture medium... More

关键词

2,4,6-trihydroxybenzophenone, Escherichia coli, benzoate-CoA ligase, benzophenone synthase, biotransformation, sodium benzoate, structural annotation