Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | The construct encoding BriSΔ was synthesized at Genscript (Genscript Inc, New Jersey USA) followed by cloning into pACEBac1 plasmid (Geneva Biotech, Switzerland). | Get A Quote |
As the global burden of SARS-CoV-2 infections escalates, so does the evolution of viral variants with increased transmissibility and pathology. In addition to this entrenched diversity, RNA viruses can also display genetic diversity within single infected hosts with co-existing viral variants evolving differently in distinct cell types. The BriSΔ variant, originally identified as a viral subpopulation from SARS-CoV-2 isolate hCoV-19/England/02/2020, comprises in the spike an eight amino-acid deletion encompassing a furin recognition motif and S1/S2 cleavage site. We elucidate the structure, function and molecular dynamics of this spike providing mechanistic insight into how the deletion correlates to viral cel... More