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DNA Sequencing> | … Here by using different chemical inhibitors and CRISPR/Cas9 targeting the major protein in clathrin coat … Codon-optimized spike of SARS-CoV-2 was purchased from GenScript and subcloned to pcDNA3.1. All constructs were confirmed by sequencing … | Get A Quote |
The recent COVID-19 pandemic poses a global health emergency. Cellular entry of the causative agent SARS-CoV-2 is mediated by its spike protein interacting with cellular receptor-human angiotensin converting enzyme 2 (ACE2). Here, by using lentivirus based pseudotypes bearing spike protein, we demonstrated that entry of SARS-CoV-2 into host cells was dependent on clathrin-mediated endocytosis, and phosphoinositides played essential roles during this process. In addition, we showed that the intracellular domain and the catalytic activity of ACE2 were not required for efficient virus entry. Finally, we showed that the current predominant Delta variant, although with high infectivity and high syncytium formation, ... More