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Catalog Peptides> | … After an initial 1.5 h of BFA treatment, 0.5 mg/ml purified Gag293 peptide (FRDYVDRFYKTLRAEQASQE) (Genscript) was added to the appropriate wells of uninfected DCs and T CD4+ s… | Get A Quote |
HIV-1-specific CD4 T cells (Ts) play a critical role in controlling HIV-1 infection. Canonically, Ts are activated by peptides derived from extracellular ("exogenous") Ags displayed in complex with MHC class II (MHC II) molecules on the surfaces of "professional" APCs such as dendritic cells (DCs). In contrast, activated human Ts, which express MHC II, are not typically considered for their APC potential because of their low endocytic capacity and the exogenous Ag systems historically used for assessment. Using primary Ts and monocyte-derived DCs from healthy donors, we show that activated human Ts are highly effective at MHC II-restricted presentation of an immunodominant HIV-1-derived epitope postinfection an... More