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PCR Cloning and Subcloning> | … (+)-MlLuc1.1 plasmid was constructed by GenScript Biotech Corporation, subcloning the full-length MlLuc1.1 cDNA sequence into the HindIII/EcoRI site. U87MG cells were grown to 60–… | Get A Quote |
The development of new diagnostic tools in tumor pathology allows the optimization of individualized therapies in cancer patients. The functional optical image provides a unique opportunity to identify the pathophysiological characteristics of each tumor in a non-invasive way. Although fluorescent recombinant affibodies and nanobodies, capable of detecting certain membrane proteins present in tumor cells, has been described, the use of bioluminescent molecules is gaining a great impact in this field due to its high sensitivity. In this work, we characterize a new luciferase from the Metridia lucens copepod (MlLuc) and develop a novel bioluminescent recombinant affibody (MlLuc-aff) capable of recognizing the HER... More