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Bisulfite Amplicon Sequencing Can Detect Glia and Neuron Cell-Free DNA in Blood Plasma

Front Mol Neurosci. 2021-07; 
Zac Chatterton, Natalia Mendelev, Sean Chen, Walter Carr, Gary H Kamimori, Yongchao Ge, Andrew J Dwork, Fatemeh Haghighi
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摘要

Sampling the live brain is difficult and dangerous, and withdrawing cerebrospinal fluid is uncomfortable and frightening to the subject, so new sources of real-time analysis are constantly sought. Cell-free DNA (cfDNA) derived from glia and neurons offers the potential for wide-ranging neurological disease diagnosis and monitoring. However, new laboratory and bioinformatic strategies are needed. DNA methylation patterns on individual cfDNA fragments can be used to ascribe their cell-of-origin. Here we describe bisulfite sequencing assays and bioinformatic processing methods to identify cfDNA derived from glia and neurons. In proof-of-concept experiments, we describe the presence of both glia- and neuron-cfDNA i... More

关键词

cfDNA, diagnostic, epigenetic, glia, neuron, neurotrauma