Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | … The goat anti-mouse IgG (H+ L), protein A resin, and protein L resin were ordered from GenScript (Nanjing, China). The mouse monoclonal antibody isotyping kit was purchased from … | Get A Quote |
Sampling the live brain is difficult and dangerous, and withdrawing cerebrospinal fluid is uncomfortable and frightening to the subject, so new sources of real-time analysis are constantly sought. Cell-free DNA (cfDNA) derived from glia and neurons offers the potential for wide-ranging neurological disease diagnosis and monitoring. However, new laboratory and bioinformatic strategies are needed. DNA methylation patterns on individual cfDNA fragments can be used to ascribe their cell-of-origin. Here we describe bisulfite sequencing assays and bioinformatic processing methods to identify cfDNA derived from glia and neurons. In proof-of-concept experiments, we describe the presence of both glia- and neuron-cfDNA i... More