Products/Services Used | Details | Operation |
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Mutagenesis Services> | … synthesized and cloned into rA1-nCas9 architecture by Genscript Biotech (Nanjing). Plasmid site-directed mutagenesis was performed using the Fast Site-Directed Mutagenesis Kit (… | Get A Quote |
CRISPR/Cas9 system is a robust genome editing platform in biotechnology and medicine. However, it generally produces small insertions/deletions (indels, typically 1-3 bp) but rarely induces larger deletions in specific target sites. Here, we report a cytidine deaminase-Cas9 fusion-induced deletion system (C-DEL) and an adenine deaminase-Cas9 fusion-induced deletion system (A-DEL) by combining Cas9 with rat APOBEC1 (rA1) and TadA 8e, respectively. Both C-DEL and A-DEL improve the efficiency of deletions compared with the conventional Cas9 system in human cells. In addition, the C-DEL system generates a considerable fraction of predictable multinucleotide deletions from 5'-deaminated C bases to the Cas9-cleavage ... More