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Characteristics of NtCCD1-3 from tobacco, and protein engineering of the CCD1 to enhance -ionone production in yeast

Front Microbiol. 2022-09; 
Xiaowei Gong, Fan Li, Yupeng Liang, Xiulin Han, Mengliang Wen
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Custom Vector Construction … Pos5 gene was codon-optimized and synthesized by GenScript (Nanjing, China) and then cloned into the vector tpLADH1 to acquire tpLADH1-Pos5. The P TEF1 -Pos5-T ADH1 and P … Get A Quote

摘要

Biosynthesis of -ionone by microbial cell factories has become a promising way to obtain natural -ionone. The catalytic activity of carotenoid cleavage dioxygenase 1 (CCD1) in cleavage of -carotene to -ionone severely limits its biosynthesis. In this study, NtCCD1-3 from with high ability to cleave -carotene was screened. Multiple strategies for improving the -ionone yield in were performed. The results showed that NtCCD1-3 could cleave a variety of caroteniods at the 9,10 (9',10') double bonds and lycopene at the 5,6 (5',6') positions. The insertion site delta for gene was more suitable for enhancing the yield of -ionone, showing 19.1-fold increase compared with the rox1 site. More importantly, mutant K38A ... More

关键词

Nicotiana tabacum, Saccharomyces cerevisiae, carotenoid cleavage dioxygenase 1, protein engineering, β-ionone