Products/Services Used | Details | Operation |
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Catalog Peptides> | … Peptides corresponding to individual E1A or E2F2 binding motifs were synthesized by FMoc chemistry at >95% purity (GenScript) and quantified by Absorbance at 280 nm or by … | Get A Quote |
Many disordered proteins conserve essential functions in the face of extensive sequence variation, making it challenging to identify the mechanisms responsible for functional selection. Here we identify the molecular mechanism of functional selection for the disordered adenovirus early gene 1A (E1A) protein. E1A competes with host factors to bind the retinoblastoma (Rb) protein, subverting cell cycle regulation. We show that two binding motifs tethered by a hypervariable disordered linker drive picomolar affinity Rb binding and host factor displacement. Compensatory changes in amino acid sequence composition and sequence length lead to conservation of optimal tethering across a large family of E1A linkers. We r... More