Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … All recombinant plasmids were synthesized by GenScript … HisTrapTM column (GE healthcare) to purify these NPs. The … We obtained the unfolding profiles of freshly purified 3MCD-f … | Get A Quote |
Currently, the incorporation of multiple epitopes into vaccines is more desirable than the incorporation of a single antigen for universal influenza vaccine development. However, epitopes induce poor immune responses. Although the use of adjuvants can overcome this obstacle, it may raise new problems. Effective antigen delivery vehicles that can function as both antigen carriers and intrinsic adjuvants are highly desired for vaccine development. Here, we report a biepitope nanovaccine that provides complete protection in mice against H3N2 virus as well as partial protection against H1N1 virus. This vaccine (3MCD-f) consists of two conserved epitopes (matrix protein 2 ectodomain (M2e) and CDhelix), and these epi... More