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Plasmid DNA Preparation> | … First, a sequence-verified HDR donor plasmid was designed and synthesized (Genscript, Piscataway, NJ, USA), including the mEGFP coding sequence flanked by 230 bp homology … | Get A Quote |
The potential of human-induced pluripotent stem cells (hiPSCs) to be differentiated into cardiomyocytes (CMs) mimicking adult CMs functional morphology, marker genes and signaling characteristics has been investigated since over a decade. The evolution of the membrane localization of CM-specific G protein-coupled receptors throughout differentiation has received, however, only limited attention to date. We employ here advanced fluorescent spectroscopy, namely linescan Fluorescence Correlation Spectroscopy (FCS), to observe how the plasma membrane abundance of the β- and β-adrenergic receptors (β-ARs), labelled using a bright and photostable fluorescent antagonist, evolves during the long-term monolayer cultu... More