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Custom Vector Construction> | … , they may have slower reaction rates, allowing us to trap reaction intermediates. … coli overexpression, was synthesized by Genscript and sub-cloned into pASK-IBA3+ vector. OvoA Mtht … | Get A Quote |
Mononuclear non-heme iron enzymes are a large class of enzymes catalyzing a wide-range of reactions. In this work, we report that a non-heme iron enzyme in , OvoA has two different activities, as a thiol oxygenase and a sulfoxide synthase. When cysteine is presented as the only substrate, OvoA is a thiol oxygenase. In the presence of both histidine and cysteine as substrates, OvoA catalyzes the oxidative coupling between histidine and cysteine (a sulfoxide synthase). Additionally, we demonstrate that both substrates and the active site iron's secondary coordination shell residues exert exquisite control over the dual activities of OvoA (sulfoxide synthase thiol oxygenase activities). OvoA is an excellent syste... More