Products/Services Used | Details | Operation |
---|---|---|
Mutagenesis Services> | … Incubation of OXA-48 with CS 2 , SCNH and OCNH showed us … Given these observations it was surprising to us that, … site-directed mutagenesis by GenScript. Purified Spinacia oleracea … | Get A Quote |
Carbon dioxide is an omnipresent gas that drives adaptive responses within organisms from all domains of life. The molecular mechanisms by which proteins serve as sensors of CO are, accordingly, of great interest. Because CO is electrophilic, one way it can modulate protein biochemistry is by carboxylation of the amine group of lysine residues. However, the resulting CO-carboxylated lysines spontaneously decompose, giving off CO, which makes studying this modification difficult. Here we describe a method to stably mimic CO-carboxylated lysine residues in proteins. We leverage this method to develop a quantitative approach to identify CO-carboxylated lysines of proteins and explore the lysine 'carboxylome' of th... More