Products/Services Used | Details | Operation |
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Recombinant Antibody Expression> | … fusion gene was ordered from Genscript (Piscataway, New … construct we produced a purified master virus seed 170 stock … 178 in wells treated with the purified recombinant devil IFN-γ. … | Get A Quote |
The devil facial tumour disease (DFTD) has led to a massive decline in the wild Tasmanian devil () population. The disease is caused by two independent devil facial tumours (DFT1 and DFT2). These transmissible cancers have a mortality rate of nearly 100 %. An adenoviral vector-based vaccine has been proposed as a conservation strategy for the Tasmanian devil. This study aimed to determine if a human adenovirus serotype 5 could express functional transgenes in devil cells. As DFT1 cells do not constitutively express major histocompatibility complex class I (MHC-I), we developed a replication-deficient adenoviral vector that encodes devil interferon gamma (IFN-γ) fused to a fluorescent protein reporter. Our re... More