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A human adenovirus encoding IFN-γ can transduce Tasmanian devil facial tumour cells and upregulate MHC-I

J Gen Virol. 2022-11; 
Ahab N Kayigwe, Jocelyn M Darby, A Bruce Lyons, Amanda L Patchett, Leszek Lisowski, Guei-Sheung Liu, Andrew S Flies
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Recombinant Antibody Expression … fusion gene was ordered from Genscript (Piscataway, New … construct we produced a purified master virus seed 170 stock … 178 in wells treated with the purified recombinant devil IFN-γ. … Get A Quote

摘要

The devil facial tumour disease (DFTD) has led to a massive decline in the wild Tasmanian devil () population. The disease is caused by two independent devil facial tumours (DFT1 and DFT2). These transmissible cancers have a mortality rate of nearly 100 %. An adenoviral vector-based vaccine has been proposed as a conservation strategy for the Tasmanian devil. This study aimed to determine if a human adenovirus serotype 5 could express functional transgenes in devil cells. As DFT1 cells do not constitutively express major histocompatibility complex class I (MHC-I), we developed a replication-deficient adenoviral vector that encodes devil interferon gamma (IFN-γ) fused to a fluorescent protein reporter. Our re... More

关键词

MHC-I, Tasmanian devil, adenovirus, interferon, oral bait vaccine, wildlife vaccines