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N-gene-complementary antisense-oligonucleotide directed molecular aggregation of dual-colour carbon dots, leading to efficient fluorometric sensing of SARS-COV-2 RNA

Nanoscale. 2022-03; 
Zach Sheffield, Maha Alafeef, Parikshit Moitra, Priyanka Ray, Dipanjan Pan
Products/Services Used Details Operation
Custom DNA/RNA Oligos … The amine-modified antisense oligonucleotides (ASO) were procured from GenScript and stored at −20 C. A purified RNA extraction kit was purchased from Invitrogen. All the other … Get A Quote

摘要

The early stages of the COVID-19 pandemic punctuated the need for rapid, mass testing for early detection of viral infection. Carbon dots are easily synthesized, cost-effective fluorescent nanoparticles whose surface functionalities enable facile conjugation with biorecognition elements suitable for  molecular detection of viral RNA. Herein, we report that a pair of complementary antisense oligonucleotide (ASO) sequences can lead to a highly specific molecular aggregation of dual colour carbon dots (CDs) in the presence of SARS-CoV-2 RNA. The nanoprobes used ASOs highly specific to the N-gene of SARS-COV-2. When the ASOs are conjugated to blue and yellow citric acid-derived CDs, the combination of the ASO-CD p... More

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