Products/Services Used | Details | Operation |
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Custom Vector Construction> | … (Genscript), and cloned into pcDNA™3.1/Zeo (+) expression vector (Thermo Fisher Scientific). Trimeric SIV GP140 proteins were produced by transient transfection of FreeStyle™ 293-F … | Get A Quote |
HIV infection induces tissue damage including lymph node (LN) fibrosis and intestinal epithelial barrier disruption leading to bacterial translocation and systemic inflammation. Natural hosts of SIV, such as African Green Monkeys (AGM), do not display tissue damage despite high viral load in blood and intestinal mucosa. AGM mount a NK cell-mediated control of SIVagm replication in peripheral LN. We analyzed if NK cells also control SIVagm in mesenteric (mes) LN and if this has an impact on gut humoral responses and the production of IgA known for their anti-inflammatory role in the gut. We show that CXCR5 + NK cell frequencies increase in mesLN upon SIVagm infection and that NK cells migrate into and contro... More