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Custom Vector Construction> | … injected into tobacco leaves at a ratio of 1:10. In addition, Agrobacterium cells containing reporter construct and empty vector were injected at … were synthesized by GenScript (China) to … | Get A Quote |
WRKY transcription factors play important roles in abiotic stress by directly regulating stress-related genes. However, the molecular mechanism of its involvement in salt stress in pak-choi is still poorly understood. In this study, we elucidated the function of BcWRKY1 from pak-choi (Brassica rapa ssp. chinensis) in salt stress. The expression level of BcWRKY1 showed the highest in rosette leaves among different tissues and was induced by salt and ABA treatment in pak-choi. Subcellular localization showed that BcWRKY1 was located in nucleus. The transgenic Arabidopsis overexpressing BcWRKY1 exhibited enhanced salt sensitivity and higher HO contents, which were further confirmed by silencing BcWRKY1 in pak-choi... More