Products/Services Used | Details | Operation |
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Catalog Peptides> | … Splenocytes (5 × 10 5 or 1 × 10 6 cells) from vaccinated mice were added to each 96-well and stimulated with 1 μg SARS-CoV-2 spike glycoprotein peptide pool (GenScript Biotech). … | Get A Quote |
Coronavirus disease 2019 caused by the novel human severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently a major threat to public health worldwide. To deal with the needs of vaccine, we developed four DNA vaccine candidates against SARS-CoV-2, based on the full-length spike (S) or truncated S protein. Following mice vaccination, we measured T-cell response and antigen-specific neutralizing antibody (NAb) titer. All four candidates induced humoral immune responses, including elevated levels of total IgG and NAbs, and cell-mediated immune responses, including multiple cytokine expression. However, the full-length S DNA vaccine enhanced the immune responses most significantly. We then evaluated... More