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Custom Vector Construction> | … All peptide reactivities were verified using an independently made HPLC purified peptide. In … and cloned into the MSGV1 retroviral vector (GenScript). For TCRs with two putative TCR-… | Get A Quote |
Tumor-infiltrating neoantigen-reactive T cells can mediate regression of metastatic gastrointestinal cancers yet remain poorly characterized. We performed immunological screening against personalized neoantigens in combination with single-cell RNA sequencing on tumor-infiltrating lymphocytes from bile duct and pancreatic cancer patients to characterize the transcriptomic landscape of neoantigen-reactive T cells. We found that most neoantigen-reactive CD8 T cells displayed an exhausted state with significant CXCL13 and GZMA co-expression compared with non-neoantigen-reactive bystander cells. Most neoantigen-reactive CD4 T cells from a patient with bile duct cancer also exhibited an exhausted phenotype but wi... More