Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … injection into the tail vein, fluorescence signal was detected in the vasculature of the brain (Figure 2b, lower middle panel). By 4 h post injection… plasmid vector by Genscript. CTX vector, … | Get A Quote |
M13 bacteriophage (phage) are versatile, genetically tunable nanocarriers that have been recently adapted for use as diagnostic and therapeutic platforms. Applying p3 capsid chlorotoxin fusion with the "" circular single-stranded DNA (cssDNA) gene packaging system, we produced miniature chlorotoxin (CTX-) phage particles with a minimum length of 50 nm that can target intracranial orthotopic patient-derived GBM22 glioblastoma tumors in the brains of mice. Systemically administered indocyanine green conjugated CTX- phage accumulated in brain tumors, facilitating shortwave infrared detection. Furthermore, we show that our phage can carry cssDNA that are transcriptionally active when delivered to GBM22 glioma cel... More