Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … purchased from GenScript (US distribution, Piscataway, NJ). This plasmid was transformed into BL21(DE3) E. coli cells (New England Biolabs, Ipswich, MA) using standard protocols. … | Get A Quote |
High resolution hydroxyl radical protein footprinting (HR-HRPF) is a mass spectrometry-based method that measures the solvent exposure of multiple amino acids in a single experiment, offering constraints for experimentally informed computational modeling. HR-HRPF-based modeling has previously been used to accurately model the structure of proteins of known structure, but the technique has never been used to determine the structure of a protein of unknown structure. Here, we present the use of HR-HRPF-based modeling to determine the structure of the Ig-like domain of NRG1, a protein with no close homolog of known structure. Independent determination of the protein structure by both HR-HRPF-based modeling and het... More