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Nuclear morphogenesis: forming a heterogeneous nucleus during embryogenesis

Development. 2022-02; 
Albert Tsai, Justin Crocker
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Plasmid DNA Preparation … The plasmids were synthesized by Genscript. The constructs were injected into Bloomington Drosophila Stock Center line 27388 and integrated using RMCE by GenetiVision. Virgin … Get A Quote

摘要

An embryo experiences increasingly complex spatial and temporal patterns of gene expression as it matures, guiding the morphogenesis of its body. Using super-resolution fluorescence microscopy in Drosophila melanogaster embryos, we observed that the nuclear distributions of transcription factors and histone modifications undergo a similar transformation of increasing heterogeneity. This spatial partitioning of the nucleus could lead to distinct local regulatory environments in space and time that are tuned for specific genes. Accordingly, transcription sites driven by different cis-regulatory regions each had their own temporally and spatially varying local histone environments, which could facilitate the finer... More

关键词

Drosophila melanogaster, Biophysics, Nuclear organization, Super-resolution fluorescence imaging