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Custom DNA/RNA Oligos> | … Ser561 to Asn and Ser562 to Thr by co-injecting the gRNA and the donor oligo containing p.… -optimized human Appbp2 cDNA were synthesized by GenScript and cloned into a PMSCV-… | Get A Quote |
Compelling evidence shows that brown and beige adipose tissue are protective against metabolic diseases. PR domain-containing 16 (PRDM16) is a dominant activator of the biogenesis of beige adipocytes by forming a complex with transcriptional and epigenetic factors and is therefore an attractive target for improving metabolic health. However, a lack of knowledge surrounding the regulation of PRDM16 protein expression hampered us from selectively targeting this transcriptional pathway. Here we identify CUL2-APPBP2 as the ubiquitin E3 ligase that determines PRDM16 protein stability by catalysing its polyubiquitination. Inhibition of CUL2-APPBP2 sufficiently extended the half-life of PRDM16 protein and promoted be... More