Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | … -GMP-acarbose structure obtained at pH 8.5 allowed us to capture a covalent intermediate 38 . … coli expression, purchased from Genscript Corporation and subcloned into pET15b such … | Get A Quote |
Streptomyces are our principal source of antibiotics, which they generate concomitant with a complex developmental transition from vegetative hyphae to spores. c-di-GMP acts as a linchpin in this transition by binding and regulating the key developmental regulators, BldD and WhiG. Here we show that c-di-GMP also binds the glycogen-debranching-enzyme, GlgX, uncovering a direct link between c-di-GMP and glycogen metabolism in bacteria. Further, we show c-di-GMP binding is required for GlgX activity. We describe structures of apo and c-di-GMP-bound GlgX and, strikingly, their comparison shows c-di-GMP induces long-range conformational changes, reorganizing the catalytic pocket to an active state. Glycogen is an im... More