Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | … The gene encoding Omicron S protein was purchased from Genscript and then subcloned … 100 μg of antibodies in 500 μL of injection volume was chromatographed onto the Superdex… | Get A Quote |
The effectiveness of SARS-CoV-2 vaccines and therapeutic antibodies have been limited by the continuous emergence of viral variants and by the restricted diffusion of antibodies from circulation into the sites of respiratory virus infection. Here, we report the identification of two highly conserved regions on the Omicron variant receptor-binding domain recognized by broadly neutralizing antibodies. Furthermore, we generated a bispecific single-domain antibody that was able to simultaneously and synergistically bind these two regions on a single Omicron variant receptor-binding domain as revealed by cryo-EM structures. We demonstrated that this bispecific antibody can be effectively delivered to lung via inhala... More