Products/Services Used | Details | Operation |
---|---|---|
Plasmid DNA Preparation> | … The gene encoding of CalB was synthesized by GenScript and inserted in the vector pET-26b (+) between the two restriction sites NdeI and XhoI. The recombinant plasmid, called pET… | Get A Quote |
In this study, lipase B (CalB) was covalently immobilized on the surface of graphene oxide (GO) nanoparticles by sortase-mediated immobilization as well as a chemical attachment approach. Sortase is a transpeptidase that provides one-step purification and targeted immobilization of CalB from one specific site, presenting oriented attachment of the enzyme to a solid support. Chemical immobilization, on the other hand, is considered as a random immobilization, in which the protein can bind to the support from different regions of the protein surface. In this approach, amine-functionalized GO was further modified with glutaraldehyde to facilitate the covalent binding of CalB via its amine residues. The applied me... More