Products/Services Used | Details | Operation |
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Custom DNA/RNA Oligos> | … and papC genes from Pseudomonas fluorescens were synthesized by GenScript (Piscataway, NJ). Primers and single-stranded oligonucleotides used for cloning and MAGE were … | Get A Quote |
Introducing heterologous pathways into host cells constitutes a promising strategy for synthesizing nonstandard amino acids (nsAAs) to enable the production of proteins with expanded chemistries. However, this strategy has proven challenging, as the expression of heterologous pathways can disrupt cellular homeostasis of the host cell. Here, we sought to optimize the heterologous production of the nsAA -aminophenylalanine (pAF) in . First, we incorporated a heterologous pAF biosynthesis pathway into a genome-scale model of metabolism and computationally identified metabolic interventions in the host's native metabolism to improve pAF production. Next, we explored different approaches of imposing these flux inte... More