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Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts

Biomed Mater. 2021-02; 
Elena Filova, Marie Steinerova, Martina Travnickova, Jarmila Knitlova, Jana Musilkova, Adam Eckhardt, Daniel Hadraba, Roman Matejka, Simon Prazak, Jana Stepanovska, Johanka Kucerova, Tomáš Riedel, Eduard Brynda, Alena Lodererova, Eva Honsova, Jan Pirk, Miroslav Konarik, Lucie Bacakova
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摘要

An ideal decellularized allogenic or xenogeneic cardiovascular graft should be capable of preventing thrombus formation after implantation. The antithrombogenicity of the graft is ensured by a confluent endothelial cell layer formed on its surface. Later repopulation and remodeling of the scaffold by the patient's cells should result in the formation of living autologous tissue. In the work presented here, decellularized porcine pericardium scaffolds were modified by growing a fibrin mesh on the surface and inside the scaffolds, and by attaching heparin and human vascular endothelial growth factor (VEGF) to this mesh. Then the scaffolds were seeded with human adipose tissue-derived stem cells (ASCs). While the ... More

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