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Alteration of Allergen Fold of Bos d 5 into a Hypoallergenic Vaccine for Immunotherapy of Cow's Milk Allergy

Int Arch Allergy Immunol. 2021-08; 
Yaqiong Cui, Yang Yu, Shuxiang Lin, Li Xu, Liuxu Li, Chunquan Cai, Huiqiang Li
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Bacterial Expression … The E. coli strain BL21 (DE3) was obtained from Genscript (Jiangsu, China). Natural Bos d 5 (nBos d 5) was purchased from Sigma-Aldrich (Saint Louis, MO, USA) … Get A Quote

摘要

background: Cow's milk allergy (CMA) is the most common IgE-mediated food allergy and Bos d 5 is the major allergen in cow's milk proteins. More than 60% of the patients with CMA are sensitized to this protein. results: A recombinant protein, encoded by a synthetic gene and consisting of reassembled Bos d 5 fragments, was expressed in E. coli strain BL21 (DE3) cells and purified to homogeneity. The B5M lacked relevant IgE-reactivity and allergenic activity compared with Bos d 5 in dot-blot and basophil activation assays. T-cell proliferation experiments demonstrated that B5M preserved the main T cell epitopes of Bos d 5. Immunization of rabbits with B5M induced protective IgG antibodies that blocked the binding... More

关键词

Allergen-specific immunotherapy, Bioinformatics, Bos d 5, Cow’s milk allergy, Epitope, Genetic engineering