Products/Services Used | Details | Operation |
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Mutagenesis Services> | … 5). Mycoplasma codon-optimised genes purchased from GenScript were used … Site-directed mutagenesis was used to generate the gene variants of cas9 (B) and cre (B and C) genes … | Get A Quote |
Mycoplasmas have exceptionally streamlined genomes and are strongly adapted to their many hosts, which provide them with essential nutrients. Owing to their relative genomic simplicity, Mycoplasmas have been used to develop chassis for biotechnological applications. However, the dearth of robust and precise toolkits for genomic manipulation and tight regulation has hindered any substantial advance. Herein we describe the construction of a robust genetic toolkit for M. pneumoniae, and its successful deployment to engineer synthetic gene switches that control and limit Mycoplasma growth, for biosafety containment applications. We found these synthetic gene circuits to be stable and robust in the long-term, in the... More