Products/Services Used | Details | Operation |
---|---|---|
Plasmid DNA Preparation> | … Materials and methods Fusion protein expression and purification. The intein-7-140 α-syn fusion protein cDNA was synthesized by GenScript and inserted into a pT7-7 plasmid … | Get A Quote |
α-Synuclein is a key protein of the nervous system, which regulates the release and recycling of neurotransmitters in the synapses. It is also involved in several neurodegenerative conditions, including Parkinson's disease and Multiple System Atrophy, where it forms toxic aggregates. The N-terminus of α-synuclein is of particular interest as it has been linked to both the physiological and pathological functions of the protein and undergoes post-translational modification. One such modification, N-terminal truncation, affects the aggregation propensity of the protein and is also found in aggregates from patients' brains. To date, our understanding of the role of this modification has been limited by the many... More