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Production of active recombinant human aldehyde oxidase (AOX) in the baculovirus expression vector system (BEVS) and deployment in a pre-clinical fraction-of-control AOX compound exposure assay

Protein Expr Purif. 2020-09; 
Ciarán N Cronin, JianHua Liu, Nicole Grable, Timothy J Strelevitz, R Scott Obach, Anthony Carlo
Products/Services Used Details Operation
Mammalian Expression … The DNA sequence encoding full-length human AOX (NM_001159.3) was synthesized at GenScript, and was modified minimally with silent mutations to remove certain restriction enzyme cloning sites (BamH I, EcoR I, Hind III and Nde I) in order to allow for sub-cloning of the … Get A Quote

摘要

Human aldehyde oxidase (AOX) has emerged as a key enzyme activity for consideration in modern drug discovery. The enzyme catalyzes the oxidation of a wide variety of compounds, most notably azaheterocyclics that often form the building blocks of small molecule therapeutics. Failure to consider and assess AOX drug exposure early in the drug development cycle can have catastrophic consequences for novel compounds entering the clinic. AOX is a complex molybdopterin-containing iron-sulfur flavoprotein comprised of two identical 150 kDa subunits that has proven difficult to produce in recombinant form, and a commercial source of the purified human enzyme is currently unavailable. Thus, the potential exposure of nov... More

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