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Mutagenesis Services> | … Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD … number MN908947.3) was obtained from GenScript. Site-directed mutagenesis was used to … | Get A Quote |
Glycan-masking the vaccine antigen by mutating the undesired antigenic sites with an additional -linked glycosylation motif can refocus B-cell responses to desired epitopes, without affecting the antigen's overall-folded structure. This study examined the impact of glycan-masking mutants of the N-terminal domain (NTD) and receptor-binding domain (RBD) of SARS-CoV-2, and found that the antigenic design of the S protein increases the neutralizing antibody titers against the Wuhan-Hu-1 ancestral strain and the recently emerged SARS-CoV-2 variants Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.2). Our results demonstrated that the use of glycan-masking Ad-S-R158N/Y160T in the NTD elicited a 2.8-fold, 6.5-fold,... More