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A single residue deletion in the barley HKT1;5 P189 variant restores plasma membrane localisation but not Na conductance

Biochim Biophys Acta Biomembr. 2021-06; 
Stefanie Wege, Jiaen Qiu, Caitlin Byrt, Kelly Houston, Robbie Waugh, Matthew Gilliham, Maria Hrmova
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摘要

Leaf Na exclusion, mediated by plasma membrane-localised Class 1 High-affinity potassium (K) Transporters (HKTs), is a key mechanism contributing to salinity tolerance of several major crop plants. We determined previously that the leucine to proline residue substitution at position 189 (L189P) in barley HvHKT1;5 disrupts its characteristic plasma membrane localisation and Na conductance. Here, we focus on a surprising observation that a single residue deletion of methionine at position 372 (M372del) within the conserved VMMYL motif in plant HKTs, restores plasma membrane localisation but not Na conductance in HvHKT1;5 P189. To clarify why the singular M372 deletion regains plasma membrane localisation, we buil... More

关键词

3D protein modelling, Confocal microscopy, Molecular dynamics simulations, Salinity, Two-Electrode Voltage-Clamp, α-Helical association and assembly pathways