Novel pentose-regulated promoter of with application in controlling heterologous gene expression

The potent promoter and its transcriptional control make a significant contribution to strain optimization. Using transcriptomebased approach, a novel pentose-regulated promoter of the xylose reductase gene () of  was identified. The promoter analysis showed that the  was tightly regulated by pentose sugars, which xylose and xylan were favorable inducers. The  function was highly efficient as compared with the maltose-inducible promoters of . It also exhibited the efficient transcription induction even though certain amounts of glucose and sucrose existed in the cultures. The expression control of  was dependent on xylose consumption capacity for fungal growth. The control mode of  offers a simple operation in simultaneous gene expression and cultivation optimization in Aspergilli. This study provides a prospective development of fungal production platform using cellulosic sugars by the xylose-utilizing strains for sustainable growing in circular economy.