Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … The customized sublibrary (plasmid pool synthesized at GenScript) lentivirus was prepared using the same protocol as the TKOv3 library. Individual SLIT2 CRISPR KO lentivirus was … | Get A Quote |
Circulating tumor cells (CTCs) break free from primary tumors and travel through the circulation system to seed metastatic tumors, which are the major cause of death from cancer. The identification of the major genetic factors that enhance production and persistence of CTCs in the bloodstream at a whole genome level would enable more comprehensive molecular mechanisms of metastasis to be elucidated and the identification of novel therapeutic targets, but this remains a challenging task due to the heterogeneity and extreme rarity of CTCs. Here, we describe an in vivo genome-wide CRISPR knockout screen using CTCs directly isolated from a mouse xenograft. This screen elucidated -a gene encoding a secreted protein ... More