Products/Services Used | Details | Operation |
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Plasmid DNA Preparation> | … The codon optimized HA and NA genes from the strains were synthesized (Genscript). The HA genes were separately cloned into the pCDNA5/TO (puro) plasmid and named “pcDNA5/… | Get A Quote |
Novel cell-based assays were developed to assess antibody-dependence cellular cytotoxicity (ADCC) antibodies against both vaccine and a representative circulation strain HA and NA proteins for the 2014-15 influenza season. The four assays using target cells stably expressing one of the four proteins worked well. In pre- and post-vaccine sera from 70 participants in a pre-season vaccine trial, we found ADCC antibodies and a rise in ADCC antibody titer against target cells expressing the 4 proteins but a much higher titer for the vaccine than the circulating HA in both pre-and post-vaccine sera. These differences in HA ADCC antibodies were not reflected in differences in HA binding antibodies. Our observations su... More