Products/Services Used | Details | Operation |
---|---|---|
PCR Cloning and Subcloning> | … As a resource to the biological community, we created enhancer sequences for all the … All synthesis and subcloning of plasmids was done by Genscript following our subcloning … | Get A Quote |
To express transgenes in specific cell types and states, promoters for endogenous genes are commonly created by truncating the sequence upstream of the transcriptional start site until the promoter is no longer functional. In this paper, we developed a method to design shorter synthetic mammalian promoters for endogenous genes by concatenating only its highly palindromic subsequences with a minimal core promoter. After developing metrics for palindromic density, analysis across all the human and mouse promoters showed higher palindromic density than expected by random. As experimental demonstrations, we applied the method to the CMV promoter (reduced to 432 nucleotides) and the mouse synapsin-1 promoter (383 nu... More