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Design of Synthetic Mammalian Promoters Using Highly Palindromic Subsequences

ACS Synth Biol. 2022-02; 
Polina Govorkova, Chee Ka Candice Lam, Kevin Truong
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摘要

To express transgenes in specific cell types and states, promoters for endogenous genes are commonly created by truncating the sequence upstream of the transcriptional start site until the promoter is no longer functional. In this paper, we developed a method to design shorter synthetic mammalian promoters for endogenous genes by concatenating only its highly palindromic subsequences with a minimal core promoter. After developing metrics for palindromic density, analysis across all the human and mouse promoters showed higher palindromic density than expected by random. As experimental demonstrations, we applied the method to the CMV promoter (reduced to 432 nucleotides) and the mouse synapsin-1 promoter (383 nu... More

关键词

palindrome, palindromic density graph, promoter design, synthetic promoters