Products/Services Used | Details | Operation |
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DNA Sequencing> | Modifications were verified by Sanger sequencing (Eurofins Genomics). The pLVX-CMV-XRCC1-gRNA-res-Neo vector was created by purchasing a pENTR-XRCC1-gRNA-Res construct (Genscript USA, Inc.) | Get A Quote |
Assembly and disassembly of DNA repair protein complexes at DNA damage sites are essential for maintaining genomic integrity. Investigating factors coordinating assembly of the base excision repair (BER) proteins DNA polymerase β (Polβ) and XRCC1 to DNA lesion sites identifies a role for Polβ in regulating XRCC1 disassembly from DNA repair complexes and, conversely, demonstrates Polβ's dependence on XRCC1 for complex assembly. LivePAR, a genetically encoded probe for live-cell imaging of poly(ADP-ribose) (PAR), reveals that Polβ and XRCC1 require PAR for repair-complex assembly, with PARP1 and PARP2 playing unique roles in complex dynamics. Further, BER complex assembly is modulated by attenuation/augmenta... More