至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

association with host mitochondria requires key mitochondrial protein import machinery

Proc Natl Acad Sci U S A. 2021-03; 
Matthew L Blank, Jing Xia, Mary M Morcos, Mai Sun, Pamela S Cantrell, Yang Liu, Xuemei Zeng, Cameron J Powell, Nathan Yates, Martin J Boulanger, Jon P Boyle
Products/Services Used Details Operation
Insect Expression Clones encoding amino acids T159 to D435 of TgMAF1a and S173 to D443 of TgMAF1b were codon-optimized for expression in Escherichia coli and synthesized by GenScript. ...Clones encoding amino acids N109 to A598 of human TOM70 (accession no. NP_055635.3) were codon-optimized for expression in insect cells and synthesized by GenScript Get A Quote

摘要

Host mitochondrial association (HMA) is a well-known phenomenon during infection of the host cell. The locus () is required for HMA and encodes distinct paralogs of secreted dense granule effector proteins, some of which mediate the HMA phenotype (MAF1b paralogs drive HMA; MAF1a paralogs do not). To identify host proteins required for MAF1b-mediated HMA, we performed unbiased, label-free quantitative proteomics on host cells infected with type II parasites expressing MAF1b, MAF1a, and an HMA-incompetent MAF1b mutant. Across these samples, we identified ∼1,360 MAF1-interacting proteins, but only 13 that were significantly and uniquely enriched in MAF1b pull-downs. The gene products include multiple mitocho... More

关键词

Toxoplasma gondii, mitochondria, neofunctionalization, tandem gene expansion, virulence