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Signal transduction through Cys-loop receptors is mediated by the nonspecific bumping of closely apposed domains

Proc Natl Acad Sci U S A. 2021-04; 
Gisela D Cymes, Claudio Grosman
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Custom Vector Construction elegans and the TMD of α7-AChR from chicken in pcDNA3.1 was synthesized by Genscript and was subcloned into the pMT3 vector; Get A Quote

摘要

One of the most fundamental questions in the field of Cys-loop receptors (pentameric ligand-gated ion channels, pLGICs) is how the affinity for neurotransmitters and the conductive/nonconductive state of the transmembrane pore are correlated despite the ∼60-Å distance between the corresponding domains. Proposed mechanisms differ, but they all converge into the idea that interactions between wild-type side chains across the extracellular-transmembrane-domain (ECD-TMD) interface are crucial for this phenomenon. Indeed, the successful design of fully functional chimeras that combine intact ECD and TMD modules from different wild-type pLGICs has commonly been ascribed to the residual conservation of sequence tha... More

关键词

alpha-7 nicotinic acetylcholine receptor, chimeric constructs, glutamate-gated chloride channel, ion-channel gating, pentameric ligand-gated ion channels