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Mammalian Expression> | To construct SARS-CoV-2 vaccines using our VSV vectors, we synthetically generated sequences to express the human codon-optimized receptor-binding domain (RBD) plus the envelope (E) protein, the N-terminal containing half of the spike protein (S1 subunit), and the full-length S (SF) protein of SARS-CoV-2 (GenBank: JX869059.2) (Genscript USA Inc., Piscataway, NJ, USA) | Get A Quote |
The development of safe and effective vaccines to prevent SARS-CoV-2 infections remains an urgent priority worldwide. We have used a recombinant vesicular stomatitis virus (rVSV)-based prime-boost immunization strategy to develop an effective COVID-19 vaccine candidate. We have constructed VSV genomes carrying exogenous genes resulting in the production of avirulent rVSV carrying the full-length spike protein (SF), the S1 subunit, or the receptor-binding domain (RBD) plus envelope (E) protein of SARS-CoV-2. Adding the honeybee melittin signal peptide (msp) to the N-terminus enhanced the protein expression, and adding the VSV G protein transmembrane domain and the cytoplasmic tail (Gtc) enhanced protein incorpor... More