Products/Services Used | Details | Operation |
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PCR Cloning and Subcloning> | RRID:Addgene_8515) and pcDNA3.1-C-(k)DYK-TIFAB (murine) was purchased from GenScript and were used as templates for subcloning into BamH1/EcoRI sites of pMSCV-IRES-mCherry using in-fusion following the manufacturer's instructions (Clontech) | Get A Quote |
We previously showed stabilization of NIK-induced activation of NF-κB non-canonical signaling suppresses MLL-AF9-induced AML. In the current study, we demonstrate that deletion of NF-κB non-canonical RelB prevents the inhibitory effect of NIK stabilization in MLL-AF9 AML. Mechanistically, RelB suppresses its direct target, TIFAB, which is upregulated in human AML and correlates negatively with the survival of AML patients. Forced expression of TIFAB reverses NIK-induced impaired AML development through downregulation of RelB and upregulation of HOXA9. Consistent with upregulation of HOXA9, gene set enrichment analysis shows that forced expression of TIFAB blocks myeloid cell development, upregulates leukemia ... More