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Insect Expression> | A recombinant baculovirus contains the hr1pag1 promoter that drives a synthetic gene encoding a fusion protein comprising of mosquito cecropin B1 signal peptide (1st–24th amino acid residues, sequence ID: KJ439044.1) and an insect codon-optimized SARS-CoV-2 S1 (T22th-S680th amino acid residues of S protein, Sequence ID: MT459919.1) from pUC57-2019-nCoV-S(insect) (GenScript). | Get A Quote |
The spike (S) protein is a leading vaccine candidate against SARS-CoV-2 infection. The S1 domain of S protein, which contains a critical receptor-binding domain (RBD) antigen, potentially induces protective immunoreactivities against SARS-CoV-2. In this study, we presented preclinical evaluations of a novel insect cell-derived SARS-CoV-2 recombinant S1 (rS1) protein as a potent COVID-19 vaccine candidate. The native antigenicity of rS1 was characterized by enzyme-linked immunosorbent assay with a neutralizing monoclonal antibody targeting the RBD antigen. To improve its immunogenicity, rS1-adjuvanted with fucoidan/trimethylchitosan nanoparticles (FUC-TMC NPs) and cytosine-phosphate-guanosine-oligodeoxynucleotid... More