Products/Services Used | Details | Operation |
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Monoclonal Antibody Services> | Medium was collected after 7 days and monoclonal antibodies were purified with AmMag Protein A magnetic beads (Genscript) | Get A Quote |
The first ever US Food and Drug Administration-approved messenger RNA vaccines are highly protective against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the contribution of each dose to the generation of antibodies against SARS-CoV-2 spike (S) protein and the degree of protection against novel variants warrant further study. Here, we investigated the B cell response to the BNT162b2 vaccine by integrating B cell repertoire analysis with single-cell transcriptomics pre- and post-vaccination. The first vaccine dose elicits a recall response of IgA plasmablasts targeting the S subunit S2. Three weeks after the first dose, we observed an influx of minimally mutated IgG memory B cells that ... More