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Insulin-like growth factor 2 mRNA binding protein 2 regulates proliferation, migration and angiogenesis of keratinocytes by modulating heparanase stability

Bioengineered. 2021-11; 
Shaomin Zhi, Jun Li, Xiao Kong, Xuemei Xie, Qiangli Zhang, Guoxiang Fang
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ORF cDNA Clones/MolecularCloud and then mixed with SYBR (TaKaRa, Otsu, Japan) and primer pairs (Genscript, Nanjing, China) for qRT-PCR. Get A Quote

摘要

Wound healing is related to proliferation, migration and angiogenesis of keratinocytes. Insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) is an important N6-methyladenosine (m6A) reader, which is involved in multiple processes, including wound healing. However, the function and mechanism of IGF2BP2 in keratinocyte processes are largely uncertain. In the present study, expression levels of IGF2BP2 and heparanase (HPSE) were detected by quantitative reverse transcription polymerase chain reaction and western blotting assays. Cell proliferation was investigated with cell counting kit-8 analysis. Cell migration was determined through wound healing assay. Angiogenesis was measured by tube formation assay... More

关键词

HPSE, IGF2BP2, angiogenesis, keratinocyte, migration, proliferation, wound healing