至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Reconstitution of Caveolin-1 into Artificial Lipid Membrane: Characterization by Transmission Electron Microscopy and Solid-State Nuclear Magnetic Resonance

Molecules. 2021-10; 
Yanli Zhang, Xinyan Zhang, Wenru Kong, Shuqi Wang
Products/Services Used Details Operation
Bacterial Expression DNA gene optimized for the bacterial expression of the 178 amino acids long CAV1 α-isoform was purchased from GenScript. Get A Quote

摘要

Caveolin-1 (CAV1), a membrane protein that is necessary for the formation and maintenance of caveolae, is a promising drug target for the therapy of various diseases, such as cancer, diabetes, and liver fibrosis. The biology and pathology of caveolae have been widely investigated; however, very little information about the structural features of full-length CAV1 is available, as well as its biophysical role in reshaping the cellular membrane. Here, we established a method, with high reliability and reproducibility, for the expression and purification of CAV1. Amyloid-like properties of CAV1 and its -terminal peptide CAV1(168-178) suggest a structural basis for the short linear CAV1 assemblies that have been rec... More

关键词

caveolin scaffolding domain, caveolin-1, lipid membranes, membrane curvature, ssNMR, α-helical